According to most previously conducted research, microbial Fe (III) reduction has been caused by the interaction of NO3-. This interaction has not been thoroughly studied in experiments that were conducted using solid-phase iron oxide materials, specifically, as a source. In an experiment studying such an interaction, a bacterium called Shewanella putrefaciens 200 was grown in an environment that mimicked groundwater conditions. There was an iron-creating oxidous material called goethite in this environment as well, and this was supposed to function as the solid-phase iron oxide material.
During this experiment, the NO3- reduced the growth of the surface goethite more than it reduced the growth of other substances that served similar purposes in other such experiments, namely aqueous or mircocrystalline materials. Also, the presence of the goethite reduced the decrease in quantity of the NO3-. This experiment displays a different microbial interaction than has been typically studied when doing experiments of this nature.
D. Craig Cooper et al, Chemical and Biological Interactions during Nitrate and Goethite Reduction by Shewanella putrefaciens 200, American Society for Microbiology: Applied and Environmental Microbiology, 5 March 2003.
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